@article { author = {El-Melegy, Kh. and Abdel-Salam, A. and Abdel - Shafea, Y. and Abdel-Rhman, A. and Abdel - Shafi, Seham}, title = {Inhibition of Aflatoxin B1 Production by Bacteria}, journal = {Journal of Animal and Poultry Production}, volume = {8}, number = {5}, pages = {91-95}, year = {2017}, publisher = {Mansoura University, Faculty of Agriculture}, issn = {2090-3642}, eissn = {2090-3723}, doi = {10.21608/jappmu.2017.45783}, abstract = {Aflatoxins (AFs) contamination in food is a serious problem in the world. Aflatoxin B1 (AFB1), produced by Aspergillusflavus,is secondary metabolite, highly toxic and carcinogenic. The reduction of AFs contamination in food and feed products could be achieved by some microorganisms. In this study,Lactobacillus plantarum ATCC 14917, Lactobacillus curvatus ATCC 1136, Bacillus megateirum A.F.10, Bacillus subtitles A.F.12and commercial probiotic were used to inhibit AFB1 production on yeast extract sucrose medium (YES) and on corn. All bacterial strain inhibitedA. flavusproduction of AFB1 on YES media and corn. Also, commercial probiotics hadthe ability to inhibit A. flavus growth and its production of AFB1 on corn. The most effective bacteria wasB. megaterium A.F.10. It inhibited the fungal growth with 12 mm inhibition zone and inhibits AFB1 production 100% by HPLC on YES media. Determination of AFB1 production by HPLC on corn showed that B. megaterium inhibitedthe production by 69.81% followed by commercial probiotics (59.62%).Commercial probiotics and B. megaterium had a synergistic effect in inhibition ofAFB1 production and in vitro digestion of corn.}, keywords = {Aspergillusflavus,Aflatoxin,Lactobacilli,inhibition}, url = {https://jappmu.journals.ekb.eg/article_45783.html}, eprint = {https://jappmu.journals.ekb.eg/article_45783_6b491c6cfc11f9ccc678a3b26ce99d34.pdf} } @article { author = {El-Sayed, A. and Gad, A. and AboelEla, M. and Ashour, G.}, title = {The Effeciency of Cryotop in Vitrification of In Vitro Produced Egyptian Buffalo (Bubalus Bubalis) Embryos}, journal = {Journal of Animal and Poultry Production}, volume = {8}, number = {5}, pages = {97-100}, year = {2017}, publisher = {Mansoura University, Faculty of Agriculture}, issn = {2090-3642}, eissn = {2090-3723}, doi = {10.21608/jappmu.2017.45784}, abstract = {Embryo cryopreservation becomes a pivotal side in the shape of assisted reproductive technologies. It plays an essential role in several cases such as, embryo storing during transportation, embryo preservation for future usesand establishment ofcryobanks for endangered species and rare breeds. The presentexperiment was conducted to evaluate the cryotoleranceofin vitro produced buffalo embryosat blastocyst stage to vitrification process using cryotop. Embryos were produced using conventional in vitro fertilisation technique and had been divided into two categories: non-vitrified group, which was culturedin vitro till hatched blastocyst stage and vitrified group, which was collected at day 7 for cryopreservation. A stepwise vitrification and thawing procedures were performed using Cryotop. Survival rate of embryos was observed within 4 hours of thawing, then the ability to expand and hatch was recorded.The expansion rate was higher in control group compared to vitrified group (91.66 vs. 86.36%, respectively) with no significant differences. In both groups, all expanded blastocysts reached the hatching stage normally.Therefore, our results suggested thatCryotop is aneffective tool for cryopreservation of in vitro produced Egyptian buffalo blastocyst.}, keywords = {Buffalo,embryo,Vitrification,Cryotop,In vitro fertilisation}, url = {https://jappmu.journals.ekb.eg/article_45784.html}, eprint = {https://jappmu.journals.ekb.eg/article_45784_67460624c0a266d089840d80768891a3.pdf} }