eng
Mansoura University, Faculty of Agriculture
Journal of Animal and Poultry Production
2090-3642
2090-3723
2016-07-01
7
7
225
231
10.21608/jappmu.2016.48705
48705
Original Article
Effect of Leptin and Melatonin as Protective Additives to Tris Extender on Frozen Semen Quality of Buffalo Bulls
A. Abdel-Khalek
1
H. El-Nagar
2
Omima Ibrahim
3
Animal Production Department, Faculty of Agriculture, Mansoura University. Egypt.
Animal Production Research Institute, Agricultural Research Center, Ministry of Agriculture, Egypt.
Animal Production Research Institute, Agricultural Research Center, Ministry of Agriculture, Egypt.
This study was conducted at Animal Production Research Station, El-Gemmezah, belonging to Animal Production Research Institute, Egypt, in cooperation with Department of Animal Production, Faculty of Agriculture, Mansoura university. Aim of the current study was to evaluate the possible effects of leptin and melatonin as a protective additive in Tris-extender on frozen semen quality of buffalo bulls. Five sexually mature Egyptian buffalo bulls (aged 4 to 5 years) with an average live body weight of (450-500 kg). All bulls were fed on the same diet. Semen was collected, twice a week for 4 wk (40 ejaculates for each bulls), using artificial vagina. The collected ejaculates (mass motility of more than 70%) were pooled, evaluation, divided into 7 replicates and frozen. Semen was extended in Tris- egg yolk extender as unsupplemented (T1), or supplemented with leptin (10, 20 and 50 ng/ml in T2, T3 and T4, or melatonin (10-3M, 10-6M and 10-9M in T5, T6 and T7), respectively. Semen was diluted , equilibrated at 50C for 4 h and frozen in liquid nitrogen. After storage for 4 weeks, frozen straws were thawed at 370C for 30 sec. Semen was evaluated in post-dilution, post-equilibrated and post-thawed semen. Various sperm parameters including percentage of individual motility (IM), dead sperm (DS) and sperm abnormality (SA) were determined. In the seminal plasma of thawed semen, activity of AST, ALT and LDH was measured. Total of 12 buffalo cows in heat were inseminated by semen extended with T1, T3 and T5 to determine conception rate (CR). Pregnancy rate was measured by rectal palpation on day 50 after (AI). The obtained results show that IM and SA percentages improved (P<0.05) in T3 and T5. Percentage of DS improved (P<0.05) in all treatments as compared to control. T3 showed the best (P<0.05) results concerning sperm characteristics studied in post-diluted as compared to other additives and T1. IM, DS and SA percentages showed the best (P<0.05) results in post-equilibrated semen (T3), followed T5 in comparison with other additives and control. IM, DS and SA improved (P<0.05) in post-thawed semen diluted (T3) or T5, being better with T3 more than with T5. Enzyme activity of AST, ALT and LDH reduced (P<0.05) in seminal plasma of post-thawed semen diluted with all additives as compared to control, being the lowest (P<0.05) in T3. Buffalo cows inseminated with semen (T3) showed higher (P<0.05) CR (91.6%) than T5 (75.0%) or control (T1, 66.6%).In conclusion, supplementation of 20 ng/ml leptin and 10-3 M melatonin to the freezing semen extender improved semen quality and reduce cryodamage of the buffalo bull spermatozoa.
https://jappmu.journals.ekb.eg/article_48705_0042630d51b8776f7cbcb382d112236d.pdf
Buffalo
Semen
leptin
Melatonin
freezing process
Enzyme
eng
Mansoura University, Faculty of Agriculture
Journal of Animal and Poultry Production
2090-3642
2090-3723
2016-07-01
7
7
233
239
10.21608/jappmu.2016.48706
48706
Original Article
Evaluation of the Effect of Edta Contaminated Food on the Fertility of Male Mice
Manal Ramadan
1
Doaa Sakr
2
M. Abou-Egla
3
Naeema Noegy
4
Zoology Department, Faculty of Science, Mansoura University
Zoology Department, Faculty of Science, Mansoura University
Zoology Department, Faculty of Science, Mansoura University
Zoology Department, Faculty of Science, Mansoura University
The present study was investigated the effects of Ethylenediaminet etraacetic acid(EDTA) contaminated food manipulation on the fertility of male mice the investigtion based on evaluation of physical semencharacteristics, andmorphometrical andhistopathological studies. ofForty healthy adult male Swiss Albino mice have been divided into four groups. The first group (the control) received only standard diet while the second to the fourth groups were supplemented daily with contaminated food with different EDTA concentrations, 0.5, 1.5 and 5 g/kg of food. The samples were collected after two experimental periods (four and eight weeks). Results showed that the EDTA manipulated groups exhibited significantlydecrease of sperm parameters and testosterone concentration. Morphometric study of testis in different EDTA manipulated groups showed a significant decrease of primary spermatocytes and fertile seminiferous tubules in count as compared to control group. However, histological results showed decrement of spermatogeniclineage;declined spermatozoa formation and several necrotic areas. Also, some Sertoli cells and interstitial cells were degenerated. In addition, several blood vessels revealed dilation and congestion in between the interstitial cells. All the adverse alteration in the present results was proportional with the dose of EDTA manipulate on of mice and with the experimental period expansion. In conclusion, the adverse effects of EDTA as a chelating agent induced obviousinhibition of spermatogenic lineage and degeneration of interstitial cells leading to decrease the fertility of male mice.
https://jappmu.journals.ekb.eg/article_48706_c07c9e77f4089d77912242efc74bcea6.pdf
mice EDTA
spermatogenesis
Male infertility
Semen
eng
Mansoura University, Faculty of Agriculture
Journal of Animal and Poultry Production
2090-3642
2090-3723
2016-07-01
7
7
241
248
10.21608/jappmu.2016.48707
48707
Original Article
Hatchability Traits of Domyati Duck Eggs and Subsequent growth Performance of Ducklings As Affected By Pre-Incubation in Ovo Injection with L-Carnitine
A. Ghonim
1
Anim. Prod. Res. Institute, Agric. Res. Center, Ministry of Agric. Dokki, Giza.
The main objective of the experiment is to study the effects of pre-incubation in ovo injection with different levels of L-carnitine (L-Car) on hatchability traits of Domyati duck eggs and subsequent growth performance, carcass traits, and some blood plasma constituents of ducklings. A total of 504 eggs were obtained from Domyati duck flock with an average weight 68gm. Before incubation, eggs were randomly divided into six main groups each of 84 eggs in three replicates ( 28 eggs each). The 1st group was used as a negative control (C) without injection.The2nd group (S)was used as a positive control injected with 0.1ml saline solution (solvent). The 3rd, 4th, 5th, and 6th groups were injected with 0.1 ml saline solution contains: 2, 4, 6 or 8 mg L-Car respectively. Hatchability, early (at 10 days of incubation), late (at the end of incubation) embryonic mortality and culled ducklings percentages were calculated based on the number of the fertile eggs. A total of 180 hatched ducklings belonging to the same six experimental treatment groups were divided into six groups,(of 30ducklings in three replicate,10 ducklings each). The Live body weight, body weight gain, feed consumption , feed conversion ratio and performance index were determined. The results indicated that, injection with 6 or 8 mg L-Car had significantly (P≤0.01) increased hatchability (%)compared with the other treatment groups. Also, early and late embryonic mortality were significantly (P≤0.05)decreased by in ovo injection with L-Car at doses 6 or 8 mg and insignificantly decreased culled ducklings. A significant (P≤0.01) improvement of live body weight from hatch up to the end of experimental period(1-84 days of age) was observed. The highest values were recorded by in ovo injection with L-Car at doses 6, 8, 4, and 2 mg respectively compared to the control groups. Ducklings hatched from eggs injected with L-Car (8 or 6 mg) had significantly (P≤0.01) improved body weight gain at the period from 1-28 day and the whole experimental period from 1-84 day of age. Significant improvement of feed conversion ratio and performance index was observed as a result of in ovo injection with L-Car. The better values were recorded by 6, 8,4 and 2 mg respectively compared to the control groups. The pre incubation in ovo injection with L-Car into Domyati duck eggs at doses of 2, 4, 6 and 8 mg, resulted in significant increase (P≤0.05) in the relative weight of empty carcass, major and minor pectrolis, Thigh muscle and liver, while, the breast skin with its subc. fat, Thigh skin with its subc. fat and abdominal fat (%) were significantly decreased as compared to the control groups. Plasma triglycerides, thyroxine, (T4) and triiodothyronine (T3) levels were significantly lower (for triglycerides) and higher for T4 and T3 hormones in the L-Car treatments compared with the control groups. Blood plasma level of MDA was significantly lower of ducks that hatched from L-Car injected eggs, especially those injected with 6 or 8 mg/ egg. It is concluded that, the pre-incubation in ovo injection with 6 or 8 mg L-carnitine could be used as a tool for improving the hatchability traits of Domyati duck eggs and growth performance, carcass yield and decrease fat deposition in ducklings carcasses at marketing age. It is suggested to use in ovo injection technique immediately before incubation to reduce the negative impacts (if any) of injection during embryogenesis, and for easy handling of eggs.
https://jappmu.journals.ekb.eg/article_48707_fc86050d09bc1a6b0d4fd8349c7e18e7.pdf
L-carnitine- in ovo injection- pre-incubation- performance- Carcass- Blood plasma T3
T4
MDA- Ducks
eng
Mansoura University, Faculty of Agriculture
Journal of Animal and Poultry Production
2090-3642
2090-3723
2016-07-01
7
7
249
253
10.21608/jappmu.2016.48708
48708
Original Article
Productive and Reproductive Performances of New Zealand White Doe Rabbits as Affected by Nigella Sativa Oil Supplementation under Hot and Mild Conditions in Egypt
U. Abd El-Monem
1
B. Khalil
2
M. Abdel-Hafez
3
Department of Animal Production, Faculty of Agriculture, Zagazig University, Zagazig, Egypt.
Department of Animal Production, Faculty of Agriculture, Zagazig University, Zagazig, Egypt.
Animal Production Research Institute, Agric. Res. Center, Ministry of Agriculture, Egypt.
Aim of the current study was to evaluate the effect of nigella sativa oil (NSO) supplementation under hot and mild Egyptian conditions, on physiological thermoregulatory response, blood components, gestation length, feed and water consumption during gestation and suckling period, litter size and weight at each of birth, 21 d and 30 d (weaning) of age and mortality rate of pups from birth to 30 days of age. Thirty New Zealand White (NZW) adult doe rabbits (15 in hot season and 15 in mild season) aged 6 months and weighed 2935.4 g were used. Results showed that feed intake, water consumption, rectal temperature, respiration rate, litter size and weight at birth, 21 d, weaning, pre-weaning mortality rate were affected significantly (P<0.01), while the gestation period, serum total proteins , albumin, globulin, urea and creatinine were not affected significantly by season. Supplementation the doe rabbits diet with NSO (30 g oil / kg diet) significantly improved litter size and weight at birth (P<0.01 and P<0.05), at 21 d and at weaning (P<0.05), while pre-weaning mortality rate, gestation period, feed intake, water intake, rectal temperature, respiration rate, serum total protein, albumin, globulin, urea and creatinine concentrations in blood plasma of does were not affected.It could be concluded that, feeding doe rabbits during the hot season with dietary supplementation of 30 g Nigella sativa oil/kg diet is very effective on productive and reproductive performances of rabbits.
https://jappmu.journals.ekb.eg/article_48708_be8eb878dd37b843f8c475181da3a07a.pdf
Rabbit
Nigella Sativa
Reproduction
blood biochemicals
eng
Mansoura University, Faculty of Agriculture
Journal of Animal and Poultry Production
2090-3642
2090-3723
2016-07-01
7
7
255
263
10.21608/jappmu.2016.48710
48710
Original Article
Peripartum Beta Endorphin levels in Relation to Labor Disorders and Post-Calving Reproductive Performance of Buffaloes.
O. El-Malky
1
H. Abou El-Ghait
2
Department of buffalo Research.Animal Production Research Institute, Agricultural Research Centre, Dokki, Giza, Egypt.
Mehalat Mousa station Animal Production Research Institute, Agricultural Research Centre, Dokki, Giza, Egypt
The aim of this study was determine the relationship between plasma β-endorphin profiles in the peripheral blood circulation and cortisol concentrations during peri-partum (pre- and post-partum periods) of buffalo cows severed from dystocia, retained placenta or delayed heat (oestrus) and long calving interval in the previous parity. Blood samples were collected from 20 buffalo cow in late pregnancy period (2 months before expected parturition date) until 100 days after calving. Twenty buffalo cows were divided into four groups according to parturition disorders, 5 animals in each. Animals showed normal calving (group A), suffering from dystocia (group B), with retained placenta (group C) and having long calving interval in the previous parity (group D). At parturition, results showed that buffalo cows suffering from calving disorders groups B, C and D had higher β-endorphin concentrations than that in group A. Whereas, β-endorphin concentrations were (110.15±3.21, 124.08±2.84, 104.09±2.45 and 96.53±0.94) respectively, at one month before parturition. Average β-endorphin concentration in buffalo cows with retained placenta, dystocia and delayed heat during peri-parturient period was higher (P<0.01) than in group A. Peak of β-endorphin at the time of parturition was noticed in all buffalo groups especially in group C. Both of β-endorphin and cortisol secretion showed the concomitant trend during peri-parturient period, showing gradually decrease after parturition till two months of delivery. Finally, buffalo cows which had calving disorders showed a clear impact on blood plasma β-endorphin concentration at late pregnancy, time of delivery and post-partum periods.
https://jappmu.journals.ekb.eg/article_48710_9730c93025d8d94530f65b18c63e1969.pdf
buffaloes
Beta-endorphin
Cortisol
peri-partum
post-partum
eng
Mansoura University, Faculty of Agriculture
Journal of Animal and Poultry Production
2090-3642
2090-3723
2016-07-01
7
7
265
268
10.21608/jappmu.2016.48711
48711
Original Article
Single and Multi Traits Animal Models for some Semen Traits on Friesian Bulls
Shimma El- Komey
1
K. Hussein
2
Anas Badr
3
A. Khattab
adelkhattab@yahoo.com
4
Animal production Department, Faculty of Agriculture, Tanta University
Animal production research Institute, Ministry of Agriculture, Dokki, Cairo, Egypt.
Animal production research Institute, Ministry of Agriculture, Dokki, Cairo, Egypt.
Animal production Department, Faculty of Agriculture, Tanta University
A total of 15153 ejection semen of 61 Friesian bulls, kept at InternationalLivestockManagementTrainingCenter at Sakha Farm, belonging to Animal Production Research Institute, Ministry of Agriculture, Dokki, Cairo, Egypt, during the period from 1992 to 2000 were used in the present study. Traits studied are volume ofsemen (Vol, ml.), concentrates (Con.Mill/mil.) and livability (Liva. %) of sperm. Single and multi traits animal models were used. The model included the fixed effects of month and year of collection and the random effects of animals, permanent environmental and errors. Means of Vol, Con and Liva.were 4.53 ml, 1820 mill/ml and 68.57%,respectively. Estimate of heritability from single trait andmultiple trait analysis were similar and being0.32±0.10, 0.14±0.09 and 0.18±0.09, for Vol., Con., and Liva., respectively.Genetic and phenotypic correlations amongsementraits are positive and ranged from 0.08 to 0.67 for genetic correlations and from 0.30 to 0.80 for phenotypic correlations. Estimates of sire transmitting ability (STA)from single and multi traits animal models are similar, while the accuracy of estimates are little higher for multi traits animal models and ranged from-2.88 to 3.54 ml, forVol., and from -930 to 511 mill/mil for con., and from – 23.14 to 12.15 % for liva.,
https://jappmu.journals.ekb.eg/article_48711_cdd04a9f7a48fe56bc80e2012d8c2cc3.pdf
Semen traits
Friesian
bulls
eng
Mansoura University, Faculty of Agriculture
Journal of Animal and Poultry Production
2090-3642
2090-3723
2016-07-01
7
7
269
273
10.21608/jappmu.2016.48712
48712
Original Article
Effect of Extract Crushed Fenugreek Seeds as Feed Additive on some Blood Parameters, Milk Yield and Its Composition of Lactating Egyptian Buffaloes
A. Mahgoub
1
M. Sallam
2
Animal Production Research Institute, Ministry of Agriculture, Egypt
Miniauniversity, Faculty of Agriculture, Egypt
Milk production & composition and some blood parameters were examined in Egyptian buffaloes as affected by supplemented basal diet by crushed Fenugreek seeds. Sixteen lactating buffaloes aged 5-7 years (at the third and fifth seasons of lactation); 45 days from calving were divided into four dietary treatments, 4 animals per each. Buffaloes were fed individually on basal diet ration [concentrate feed mixture (CFM) and rice straw (RS)] according to developing of body weight and milk production during the whole experimental periods. The crushed Fenugreek seeds were given as feed additives to the basal ration at a level 100, 150 and 200 g/buffalo/ day for 2nd, 3rd and 4th treatments, respectively, while the 1st treatment considered as control.The results indicated that the dry matter intake (DMI) was significantly (P<0.05) increased in T3 and T3 than other groups. Also, crushed fenugreek seeds significantly(P<0.05) increased nutrient digestibilities (DM, OM, CP and EE). The total milk yields were 806.4, 896.0, 952.0 and 907.2 Kg, respectively. Also, the overall daily milk yield was 7.2, 8.0, 8.5 and 8.1 Kg/h/d for T1, T2, T3 and T4, respectively. Lactose percentage significantly (P<0.05) increased in treated buffaloes. However, fat percentage was significantly (P<0.05) decreased with treated buffaloes. The calculated efficiency of milk yield/DMI was increased (P<0.05) in Fenugreek treated buffaloes. The addition of crushed Fenugreek seeds increased (P<0.05) the level of blood plasma prolactin and GPT in lactating buffaloes.It could be concluded that adding fenugreek seeds to buffalo's rations at 100, 150 and 200 g /buffalo/day led to raising economic efficiency by about 6.6, 3.95 and 7.89 %, respectively and it could be recommended to improve feed efficiency, milk yield and economic efficiency.
https://jappmu.journals.ekb.eg/article_48712_9f1fc7669cc2f300ac42a3525dd869a7.pdf
Fenugreek seed
Lactating buffaloes
milk yield
Prolactin hormone
eng
Mansoura University, Faculty of Agriculture
Journal of Animal and Poultry Production
2090-3642
2090-3723
2016-07-01
7
7
275
278
10.21608/jappmu.2016.48713
48713
Original Article
Concentrations of Sodium, Potassium, Copper, Zinc and Heavy Metals in Camel Milk Reared Under Pasture and Farm Conditions in South Egypt
A. Damarany
1
Department of Animal and Poultry Production, Faculty of Agriculture and Natural Resources, Aswan University, Egypt.
The present study aimed to determine concentration of sodium, potassium, copper, zinc, lead and cadmium in camel's milk. The camels was divided into two groups, the first group reared under the traditional farms and the second reared under pasture system. The elements were determined by using atomic absorption (AA) flame spectrometry techniques. The results indicated that the overall mean ofsodium and potassium contents in camel milk was 1848.54±571.2 and 3749.90±328.9 mg/l, respectively. While, the overall mean of copper and zinc contents in camel milk was 0.065±0.02 and 0.20±0.06 mg/l, respectively. The overall mean of lead and cadmium contents in milk camel was 1.56±0.85and 0.008 ± 0.001 mg/l, respectively. Sodium content in pasture camel milk was lower (1823.59±572.7 mg/l) than farm (1873.49±578.03 mg/l), but the content of potassium was higher (3868.49±162 .75 mg/l) in farm than pasture milk (3631.32±411.52 mg/l). Content of copper in pasture milk was higher (0.07±0.02 mg/l)than the farm milk (0.06 ± 0.02 mg/l). Similar trend was observed in zinc content in pasture milk was higher (0.21±0.07 mg/l) than the farm milk (0.19 ± 0.03mg/l). Lead content was equal in pasture and the farm milk (1.54±1.1 and1.58 ± 0.45 mg/l, respectively). Cadmium content in pasture milk was higher (0.016± 0.001 mg/l) than the farm milk (0.0005 ± 0.0001 mg/l). The present results of this study indicated that marked differences in concentration of K and Cd between pasture of milk and farm milk.
https://jappmu.journals.ekb.eg/article_48713_9f1d1644950bd3315945c7276a615115.pdf
Na
K
Cu
Zn
Heavy metals
Milk
camels
eng
Mansoura University, Faculty of Agriculture
Journal of Animal and Poultry Production
2090-3642
2090-3723
2016-07-01
7
7
279
285
10.21608/jappmu.2016.48714
48714
Original Article
Genetic Evaluation for Some Productive and Reproductive Traits by Using Animal Model in A Commercial Friesian Herd in Egypt
H. Awady
hassanelawady63@yahoo.com
1
A. Abd el-Khalek
2
M. Abo Elreesh
3
Animal Production Department, Faculty of Agriculture, Kafrelsheikh University, Egypt
Animal Production Department, Faculty of Agriculture, Mansoura University, PC: 35516, Mansoura, Egypt.
Animal Production Department, Faculty of Agriculture, Mansoura University, PC: 35516, Mansoura, Egypt.
Data used in this investigation were collected from 4370 records for 874 purebred Friesian cows' daughters of 44 sires from 1985 to 2003, which belong to Shobratana Animal Production Society, located in the north part of Delta region near Tanta city in Egypt. Variance and covariance components for 305 day milk yield (305- dMY, kg), lactation length (LL, day), dry period (DP, day), days open (DO, day) and calving interval, (CI, day) were estimated with REML using an animal model (Boldman, 1955), that included the fixed effects of month and year of calving and parity of cow. Random effects were animal, direct and maternal genetic effects, maternal permanent environmental effect and random effect.Overall means of (305-dMY), LL, DP, DO and CI were 4897 kg, 327 day, 120 day, 66.3 day and 401.16 day. Month and year of calving were highly significant effect (P<0.01) for all studied traits. Direct heritability (h2a) of the mentioned traits was 0.38, 0.13, 0.14, 0.11 and 0.16, respectively. Heritabilities of maternal effects (h2m) of (305-dMY) (0.13), LL (0.18), DP (0.23), DO (0.14) and CI (0.21), respectively. Estimates of direct-maternal genetic correlations in all traits studied ranging from -0.60 to 0.74. Genetic correlations among different studied traits ranged from -0.80 to 1.00 while the phenotypic correlations among investigated traits were positive, ranging from 0.07 to 0.99.Range of predicted breeding value (PBV's) of sires were 462 kg, 1.05 d, 0.24 d, 5.93 d and 2.26 d, for 305dMY, LL, DP, DO and CI, respectively. PBV's of cows of the mentioned traits was 1123 kg, 2.92 d, 1.64 d, 5.93 d and 2.92 d respectively. Corresponding PBV's of dams for the above same traits were 510 kg, 1.42 d, 0.80 d, 7.20 d and 3.00 d, respectively. Accuracy of (PBV's) ranged from 38 to 88, 73 to 88 and 39 to 89% for sires, cows and dams, respectively; indicating the genetic improvement could be attained through any bath of them. Estimates of temporary environmental variance as a proportion of phenotypic variance for LL, DP, DO and CI ranged from 35 to 71% indicating the improvement in these traits could be achieved by improving for these temporary factors.
https://jappmu.journals.ekb.eg/article_48714_a626961a4ec3e29165708d704e51ba24.pdf
Direct and maternal genetic parameters
Breeding value
Friesian cows and Animal model