ORIGINAL_ARTICLE
EFFECT OF SOME CRYOPROTECTANTS OR THEIR COMBINATIONS ON SPERM MOTILITY AND ENZYMATIC ACTIVITY IN POST-THAWED SEMEN OF FRIESIAN BULLS.
The present study aimed to investigate the effect of various cryoprotective agents including 7% glycerol (GL), 7% dimethyl sulphoxide (DMSO), 5% ethylene glycol (EG), 5% acetamide (AC) or 5% lactamide (LA) and their combinations (3.5% GL+3.5 DMSO, 3.5% GL+2.5 EG, 3.5% GL+2.5% AC and 3.5% GL+2.5% LA) on post-thawing motility, recovery rate (freezability), acrosomal status and enzymatic activity of asprtate (AST) and alanin (ALT) transaminases, acid (ACP) and alkaline (ALP) phosphatases and lactic dehydrogenase (LDH) in post thawed semen. Semen was collected twice a week from five Friesian bulls (3-4 years old and 600-650 kg LBW) by means of an artificial vagina. Only semen with progressive motility ≥70% was pooled and used for different treatments. Semen was extended, frozen and thawed with lactose-yolk citrate extender containing different levels of cryoprotectants. Conception rate was also estimated by AI. Results revealed that semen extended with combination of 3.5% glycerol plus 3.5% DMSO showed significantly (P<0.05) the highest percentage of sperm motility (60.2%), recovery rate (75.6%) and the lowest percentage of damage acrosome (10%) as well as the lowest (P<0.05) activity of AST and ALT (16 and 7 U/109 sperm), ACP and ALK (105 and 50 U/109 sperm) and LDH (145 U/109 sperm) in post-thawed semen, respectively. On the other hand, semen extended with EG showed significantly (P<0.05) the poorest results as a single or in combinations of cryoprotectants.
In conclusion, using lactose-yolk-citrate extender containing a combination of 3.5% glycerol plus 3.5% DMSO during cryopreservation of bull semen showed the highest sperm motility, recovery rate and spermatozoa with intact acrosome in post-thawed semen, which reflected the highest conception rate of Friesian cows.
https://jappmu.journals.ekb.eg/article_86094_2aaf46c0e63d780a8064510f4b7c2f1d.pdf
2010-03-01
71
80
10.21608/jappmu.2010.86094
Friesian
Semen
motility
acrosome
cryoprotectants
freezing
thawing
M.
El-Harairy
mharairy53@yahoo.com
1
Anim. Prod. Dept., Fac. Agric., Mansoura University.
AUTHOR
A.
zeidan
2
Anim. Prod. Res. Institute, Agriculture Research Center.
AUTHOR
M.
El-Kishk
3
Anim. Prod. Res. Institute, Agriculture Research Center.
AUTHOR
ORIGINAL_ARTICLE
THE EFFECT OF SALBUTAMOL ADMINISTRATION ON PERFORMANCE, SOME BLOOD PARAMETERS AND SKELETAL MUSCLES HISTOLOGY OF NZW RABBITS
An experiment was conducted to evaluate the effect of supplementing a beta- adrenergic agonist drug (salbutamol) on growth, carcass traits and some physiological parameters in New Zealand White (NZW) rabbits. A total number of 36 unsexed 6-week-old rabbits, of a similar initial live body weight (LBW) were randomly allotted to 4 treatment groups; each of which was subdivided in to three replicates of three rabbits each. Group 1 (control) was fed a basal diet while groups 2, 3 and 4 were fed the same basal diet but injected (once/week) with salbutamol at doses of 100, 200 and 300 µg/kg LBW, respectively, for 8 weeks.
During the whole experimental period (6-14 weeks of age), salbutamol administration had no adverse effect on growth performance or carcass traits, with slight significant increase in kidney percentage for rabbits injected with a salbutamol dose of 300 µg/kg LBW. Blood plasma levels of glucose, triglycerides, total protein, albumin, globulin and T4 were not affected by salbutamol injection while creatinine and urea N concentrations were inconsistently lower in the salbutamol-treated rabbits than those of the control group. It was observed that salbutamol injection led to a significant increase in blood plasma levels of IGF-I and had a hypocholesterolemic effect compared with the control rabbits. Rabbits injected with 300 µg of salbutamol/kg LBW exhibited significantly higher blood hemoglobin concentration compared with that of the control group but other doses of salbutamol had no effect. The activity of blood plasma CPK was significantly lower with a dose-dependent trend to some extent, in the salbutamol-treated rabbits compared with that of the control group. Also, rabbits injected with 100 or 300 µg salbutamol/kg LBW showed significantly lower activities of LDH in blood plasma compared with that of the control group; however, activities of ALT and AST were not affected by salbutamol injection. On the other hand, rabbits injected with 100 µg salbutamol/kg LBW recorded significantly higher blood plasma level of T3 compared with that of the control group but other doses of salbutamol had no effect. The histological examinations indicated that salbutamol injection could improve the growth of skeletal muscles in rabbits.
Thus, it can be concluded that salbutamol injection in NZW rabbits at a dose of 100 µg/kg LBW has beneficial effects on their growth performance, muscle development and metabolic functions.
https://jappmu.journals.ekb.eg/article_86095_d42f91ed8e8285dc2a5598146ab396ff.pdf
2010-03-01
81
95
10.21608/jappmu.2010.86095
Salbutamol
performance
blood parameters
muscle growth
Rabbits
Tork
Dorra
1
Dept. Poultry Prod., Fac. Agric., Mansoura Univ., EL-Mansoura, Egypt
AUTHOR
Amina
El – Serwy
2
Dept. Poultry Prod., Fac. Agric., Mansoura Univ., EL-Mansoura, Egypt
AUTHOR
Kh
EL. Sherif
3
Dept. Poultry Prod., Fac. Agric., Mansoura Univ., EL-Mansoura, Egypt
AUTHOR
M.
Rabie
4
Dept. Poultry Prod., Fac. Agric., Mansoura Univ., EL-Mansoura, Egypt
AUTHOR
M
El-Gogary
5
Dept. Poultry Prod., Fac. Agric., Mansoura Univ., EL-Mansoura, Egypt
AUTHOR
ORIGINAL_ARTICLE
SEXUAL DESIRE, TESTICULAR MEASUREMENTS AND SEMEN QUALITY OF BUFFALO BULLS TREATED WITH COMBINATIONS OF TRACE ELEMENTS AND VITAMIN E.
A total of twenty healthy Egyptian buffalo bulls with an average of 500 kg live weight and 2.5 - 4.0 years old was used to study the effect of selenium and vitamin E (Se+Vit. E), Se and zinc (Se+Z), Vit. E+Z and Se, Vit. E+Z) on sexual desire, testicular measurements, some physical semen characteristics and liver function of buffalo bulls. The experimental animals were divided into five similar groups (4 bulls each). Bulls in the 1st group were left without any treatment and served as a control group (G1). Bulls were i.m. injected with 10 mg Se as sodium selenite and oral dose (100 IU) of Vit. E as α-tocopherol acetate per head (G2); i.m. injected with 10 mg Se and oral dose (5 g) of zinc oxide per head (G3); oral dose (100 IU) of Vit. E plus 5 g zinc oxide per head (G4); or i.m. injected with 10 mg Se and oral dose (100 IU) of Vit. E plus 5 g zinc oxide per head (G5). All treatments were twice/week for five months experimental period, two months as preliminary period and three as a main collection period. During the main collection period, sexual desire, testicular measurements, semen ejaculate volume (SEV), progressive sperm motility (PSM), sperm abnormality (SAB), sperm cell concentration (SCC) and total sperm output (TSO). Results showed that reaction time (RT) was shorter (P<0.05) and serum testosterone concentration was higher in all treated groups than in the control group. The shortest RT (68.92 sec) and the highest testosterone concentration (0.77 ng/ml) were recorded in G4. Values of testicular volume, scrotal circumference and testis tone firmer were higher (P<0.05) in all treated groups than in the control group, being the highest in G4 and the lowest in G1. Both SEV and percentage of PSM increased (P<0.05) in G2, G4 and G5 as compared to G1. The highest values of SEV and PSM were obtained for G4; however, values of G3 treated with Se+Z did not differ significantly from those in G1. Percentage of SAB reduced (P<0.05), while sperm cell concentration (SCC/ml) and total sperm output (TSO/ejaculate) increased (P<0.05) in all treated groups as compared to the control one. In conclusion, the current study may indicate beneficial effects of in vivo treatment of buffalo bulls with oral dose of Vit. E (100 IU) as α-tocopherol acetate plus 5 g zinc oxide per head twic/week for two preliminary months and three main semen collection months on sexual desire, testicular measurements and semen quality.
https://jappmu.journals.ekb.eg/article_86096_aca24b6bd71fe54a819b3e9f89b93f2a.pdf
2010-03-01
97
107
10.21608/jappmu.2010.86096
Buffalo semen
Se
vit. E
zinc
libido
testis
sperm motility
acrosome
A.
Abdel-Khalek
abdelkhalk2004@yahoo.com
1
Anim. Prod. Dept., Fac. Agric., Mansoura Univ., Egypt.
AUTHOR
M.
Yousef
2
Anim. Prod. Res. Inst., Agric. Res. Center, Egypt.
AUTHOR
A.
El-Hawary
dr.ashraf016@gmail.com
3
Anim. Prod. Res. Inst., Agric. Res. Center, Egypt.
AUTHOR