Eliraqy, E., Abd-Allah, E., Salem, M., Fouad, W. (2020). Effects of Supplementing Extenders with L-Arginine on Semen Cryopreservation and Fertility in Buffalo Bulls. Journal of Animal and Poultry Production, 11(12), 591-595. doi: 10.21608/jappmu.2020.161209
Entesar Z. Eliraqy; E. A. Abd-Allah; Mogeda M. A. Salem; W. F. M. Fouad. "Effects of Supplementing Extenders with L-Arginine on Semen Cryopreservation and Fertility in Buffalo Bulls". Journal of Animal and Poultry Production, 11, 12, 2020, 591-595. doi: 10.21608/jappmu.2020.161209
Eliraqy, E., Abd-Allah, E., Salem, M., Fouad, W. (2020). 'Effects of Supplementing Extenders with L-Arginine on Semen Cryopreservation and Fertility in Buffalo Bulls', Journal of Animal and Poultry Production, 11(12), pp. 591-595. doi: 10.21608/jappmu.2020.161209
Eliraqy, E., Abd-Allah, E., Salem, M., Fouad, W. Effects of Supplementing Extenders with L-Arginine on Semen Cryopreservation and Fertility in Buffalo Bulls. Journal of Animal and Poultry Production, 2020; 11(12): 591-595. doi: 10.21608/jappmu.2020.161209
Effects of Supplementing Extenders with L-Arginine on Semen Cryopreservation and Fertility in Buffalo Bulls
1Biotechnology Res. Dep. Animal Production Res. Institute, Agric. Res.Center, Dokki, Giza, Egypt.
2Animal Production Res. Institute, Agric. Res.Center, Dokki, Giza, Egypt.
3Cattle Breed Res. Dep. Animal Production Res. Institute, Agric. Res.Center, Dokki, Giza, Egypt.
Abstract
Knowledge of influence added more one concentration of L-arginine to enhance the poor quality of buffalo bull spermatozoa considers the main objective in our study. Semen collected from five buffalo bulls once weekly and ejaculates with 45-50% mass motility were pooled. Semen was diluted at 37C° by Tris-egg yolk extender, also separated to five portions, 1st portion without supplement it consider control to other portions and 2nd, 3rd, 4th and 5th were supplemented with 3, 5, 7 and 10 mM L-arginine respectively. Progressive sperm motility, live sperm%, abnormal sperm%, chromatin normality, Conception rate, and integrity of sperm acrosome were determined. Data declared the, extenders with concentrations of 7 and 10 mM L-Arginine was improved all parameters studied than 3 and 5 mM L-arginine extenders and control extender at different cryopreservation processes. A significant difference in chromatin integrity was detected at all treatments. Also, the conception rate was higher in buffalo cows artificially inseminated with 7 and 10 mM L-arginine (67.27 and 71.70 %) compared with control (45.45%). Conclusively, it could be concluded that adding L-arginine with concentrations of 7 or 10mM to Tris-extender improved the cryopreservation and fertility of the poor quality of Buffalo bull spermatozoa.
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