El-Nenaey,, M. (2006). 1-EFFECTS OF ANTIBODIES- ANTIGEN ON RABBITS FERTILITY. Journal of Animal and Poultry Production, 31(4), 1973-1980. doi: 10.21608/jappmu.2006.235465
M. H. El-Nenaey,. "1-EFFECTS OF ANTIBODIES- ANTIGEN ON RABBITS FERTILITY". Journal of Animal and Poultry Production, 31, 4, 2006, 1973-1980. doi: 10.21608/jappmu.2006.235465
El-Nenaey,, M. (2006). '1-EFFECTS OF ANTIBODIES- ANTIGEN ON RABBITS FERTILITY', Journal of Animal and Poultry Production, 31(4), pp. 1973-1980. doi: 10.21608/jappmu.2006.235465
El-Nenaey,, M. 1-EFFECTS OF ANTIBODIES- ANTIGEN ON RABBITS FERTILITY. Journal of Animal and Poultry Production, 2006; 31(4): 1973-1980. doi: 10.21608/jappmu.2006.235465
1-EFFECTS OF ANTIBODIES- ANTIGEN ON RABBITS FERTILITY
Animal Production Research Institute, Dokki, Giza. Egypt.
Abstract
The present study was carried out at Sakha Research Station , Kafr El —
Sheikh Governorate, belonging to Animal Production Institute (APRi), Agricultural
Research Center, Ministry of Agriculture from September to December (2005).
Eighty New Zealand White rabbits (70 does and 10 bucks) and 10
Californian bucks (CL) were randomly selected at 7 months old. Thirty were
immunized with bovine serum albumin ( BSA ), classified later into high (HR) and
low response (LR) responded groups. Ten does from each group were selected at
random. They formed with the remaining un-immunized (40) does six equal groups.
The Cior antibodies sera immunized with BSA to obtain immune sera US) or
antibodies sera (Abs), used for NZW buck semen treatments. Such treatments were
as follows; 1- Control un-immunized semen ( Culm), 2-Semen + Abs, 3- Semen +
Egg-Yolk—Tris extender (YET) + Abs, 4- Semen + (YET) + caffeine + Abs, and 5-
Semen + YET). The HR and LR and LR groups were artifically inseminated with the
control unvimmunized semen. The four un-immunized does groups were subjected to
Al. using semen from treatments 2, 3, 4, 5, respectively. insemination was
conducted after Receptal injection for does to induce ovulation. Seventy-two hours
after insemination does were dissected, and then fertilization rate and embryos
numbers were detected.
Sperm motility percentage was significantly higher (P<D.Dt) for control un-
immunized semen then semen having antibodies. Fertilization rate and total embryos
number survival embryos were the greatest for un-immunized does inseminated
with semen free of antibodies (Ps0.05). caffeine addition to the immunized semen
improved the fertilization rate and embryos survival compared with semen having
antibodies.
it could be concluded that immunized rabbits with antigens resulted in some
reproductive failures, may due to the block of binding of antibodies to these antigens,
which inhibits gamete function and compromise fertilization. The significant increase
of embryonic mortality in response Key words: Antibodies, antigen, sperm motility,
Fertility, embryonic mortality, rabbits. To the in vitro immunization of rabbit semen
was quit similar to the in vivo immunzation of rabbit does.
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