Abd El-Razek., L. (2006). EFFECT OF INJECTABLE SELENIUM ON QUALITY AND FREEZABILITY OF EGYPTIAN BUFFALO SEMEN. Journal of Animal and Poultry Production, 31(5), 2777-2785. doi: 10.21608/jappmu.2006.235491
L. M. Abd El-Razek.. "EFFECT OF INJECTABLE SELENIUM ON QUALITY AND FREEZABILITY OF EGYPTIAN BUFFALO SEMEN". Journal of Animal and Poultry Production, 31, 5, 2006, 2777-2785. doi: 10.21608/jappmu.2006.235491
Abd El-Razek., L. (2006). 'EFFECT OF INJECTABLE SELENIUM ON QUALITY AND FREEZABILITY OF EGYPTIAN BUFFALO SEMEN', Journal of Animal and Poultry Production, 31(5), pp. 2777-2785. doi: 10.21608/jappmu.2006.235491
Abd El-Razek., L. EFFECT OF INJECTABLE SELENIUM ON QUALITY AND FREEZABILITY OF EGYPTIAN BUFFALO SEMEN. Journal of Animal and Poultry Production, 2006; 31(5): 2777-2785. doi: 10.21608/jappmu.2006.235491
EFFECT OF INJECTABLE SELENIUM ON QUALITY AND FREEZABILITY OF EGYPTIAN BUFFALO SEMEN
Dept. of Anlm. Prod.. Faculty of Agric.. Kafr El-Shelkh. Tanta Univ.
Abstract
Three sexually mature and clinically normal buffalo bulls were used in this
experiment to study the effect of injectable selenium (Se) on quality. and freezability
of Egyptian buffalo semen and on plasma testosterone concentration. The bulls were
almost at the same age (3.5 years) and body weight (550 kg) at the beginning of the
experiment. The data were collected throughout a 12 weeks period. This time interval
comprised a pro-treatment period of four weeks (control) and a supplementary period
of 8 weeks. During the supplementary period. each bull was injected intramuscularly
with 10 mg Se twice weekly. The semen was collected by means of artificial vagina
twice weekly. Twenty four ejaculates collected during pro-treatment period and 4B
ejaculates collected during Se supplementation period were extended in Tris—egg
yolk-glycerol extender and packed into mini straws (0.25 ml). After 4 h equilibration at
5°C. these straws were frozen in vapour of LN; and stored for 24 h at ~196°C before
thawing and evaluation. The supplementary 86 significantly (P < 0.05) increased
ejaculate volume (2.8 vs. 2.06 ml). live sperm (69.6 vs. 61.3%). sperm concentration
(1.41 vs. 1.03 x 10°iml) and sperm output (4.0 vs. 2.11 x 10") per ejaculate and
significantly (P < 0.05) decreased sperm abnormalities (10.4 vs. 15.0%) as compared
to the pro—treatment period. In addition. treatment with injectable Se increased
fructose and Se concentrations in semen. Also, supplementary Se significantly (P <
0.05) increased blood serum testosterone (0.695 vs. 0.26 ngfml). The treatment with
So resulted in higher (P < 0.05) frozen-thawed motility and live spermatozoa
compared to the pro-treatment period. It is concluded that injection of 10 mg Se twice
weekly should be considered adequate for improvement of quality and freezability of
Egyptian buffalo semen.
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