EFFECT OF USING TWO METHODS OF STAINING ON SPERM MORPHOLOGY CHARACTERS OF BULL AND BUCK SPERM AFTER FREEZING.

Document Type : Original Article

Author

Animal Production Department, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt.

Abstract

Two different methods of staining (Harris hematoxylin&Modified Bryan's
sperm stain) were used to explore sperm morphology changes after freezing for both
bull and buck sperm. Morphology sperm characters were concerned with state of the
following sperm organelles; head, med piece, acrosome, tail and cytoplasmic droplet.
Observations of abnormal sperm morphology were higher and differed significantly for
rabbit sperm than bull sperm after freezing. In addition both bull, buck sperm
morphology differed significantly before and after freezing. It couid be concluded that,
rabbit sperm is more sensitive for freezing process than bull sperm and it is still
needed for more appropriate freezing media to avoid freezing injuries at cellular and
sub cellular levels.

Keywords