Effect of Aqueous and Methanolic Green Tea Extracts on Motility, Normal Forms, And Kinetic Parameters of Spermatozoa in Rahmani Ram Semen Equilibrated at 5 °C for 4 Hours

Document Type : Original Article

Authors

1 Animal Production Department, Faculty of Agriculture, Tanta University, Egypt.

2 Animal Production Research Institute, Agricultural Research Center, Egypt.

3 Animal Production Research Institute, Agricultural Research Center, Cairo, Egypt.

Abstract

Green tea extract is plant–derived natural antioxidant. The purpose of this study was to investigate the effect of the supplementing semen extender (Tris-citric-soybean lecithin) with different levels of aqueous (AGTE) or methanolic (MGTE) green tea extract (0.5, 1.0 and 1.5%) on the motility, livability, normality, plasma membrane integrity, and kinetic parameters of ram spermatozoa in diluted and equilibrated semen. Semen was collected from 5 rams once a week for 7 weeks using an artificial vagina. Results showed positive effect of extender containing 1% MGTE on visual motility, livability, abnormality, membrane integrity, rapid motility, sperm velocity, and normal form in post-equilibrated semen. It is possible to infer that the inclusion of methanolic green tea extract at a level of 1% in semen extender had a substantial effect on sperm quality, normality, function, and velocity of ram spermatozoa in Rahmani semen after dilution and equilibration for 4 h at 5oC.

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