IN VITRO MATURATION AND FERTILIZATION OF CRYOPRESERVED OOCYTES OF EGYPTIAN BUFFALOES.

Abdel-Khalek, A. E.; Hammad, M. E.; El-Hais, A. M. A.; Shamiah, Sh. M.; Gabr, A. A.

doi:10.21608/jappmu.2016.52723

IN VITRO MATURATION AND FERTILIZATION OF CRYOPRESERVED OOCYTES OF EGYPTIAN BUFFALOES.

Document Type : Original Article

Authors

¹ Anim. Prod. Dept., Fac. Agric., Mansoura University, Egypt.

² Anim Prod. Dept., Fac. Agric., Tanta University, Egypt.

³ Anim. Prod. Res. Institute, Agric. Res. Center, Egypt.

10.21608/jappmu.2016.52723

Abstract

The aim of this study was to evaluate the effect of cryopreservation by conventional straws on viability and normality, in vitro maturation and fertilization and blastocyst production rate of cryopreserved immature buffalo oocytes. Oocytes were recovered from ovaries of slaughtered buffaloes, cryopreserved in tissue culture medium and thawed. Thereafter, oocytes were in vitro matured, fertilized and cultured. The obtained results indicated that viability rate (89.2 vs. 92.7%) and normality percentage (67.7 vs. 80.3%) was lower (P<0.05) for the cryopreserved than fresh oocytes. Percentage of oocytes at stage (in vitro maturation rate) was lower (P<0.05) for cryopreserved than fresh oocytes (40.8 vs. 61.7%). Fertilization rate was lower (P<0.05) for cryopreserved than fresh oocytes (38.5 vs. 75.3%). Blastocyst rate relative to total oocytes was lower (P<0.05) for cryopreserved than fresh oocytes (5.2 vs. 23.6%). This study indicated the possibility of cryopreserved immature buffalo oocytes collected from ovaries of slaughtered buffaloes to in vitro mature, fertilize and develop to embryos at blastocyst stage, but at a little extend of that in fresh case.

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