EFFECT OF MATURATION MEDIA SUPPLEMENTED WITH RETINOL OR ASCORBIC ACID OR α-TOCOPHEROL ON CUMULUS CELL EXPANSION AND IN VITRO NUCLEAR MATURATION OF BUFFALO OOCYTES.

Document Type : Original Article

Authors

Animal Production Dept., Faculty of Agric., Mansoura Univ.

Abstract

The main objective of this study was to evaluate the effect of supplementing maturation media with, retinol (10 µmol/ml), ascorbic acid (100 µmol/ml) and α-tocopherol (100 µmol/ml), on cumulus cell expansion and in vitro nuclear maturation of buffalo oocytes. Oocytes were collected from ovaries taken from slaughterhouses. Only compact oocytes were used for in vitro maturation by TCM-199 medium supplemented with different vitamin levels for 22 h in CO2 incubator at 38.5 °C, 5% CO2 and 95% humidity.  Maturation rate was determined in terms of degree of expansion of cumulus cells and oocytes at metaphase-II (nuclear maturation). Results revealed that all supplements yielded higher percentages of oocytes showing full expansion of cumulus cells (77.2, 69.4 and 69.2% for tocopherol, ascorbic acid and retinol, respectively) as compared to control medium (60.2%), but the differences were not significant. Maturation rate in term of percentage of oocytes arrested at anaphase-I, telophase-I and metaphase-II was significantly higher for media supplemented with retinol, tocopherol and ascorbic acid, being 83.5, 82.2 and 81.94% as compared to 68.54% in control medium, respectively.
Conclusively, the present results may suggest that the supplementation of maturation medium (TCM-199) with retinol, ascorbic acid and α-tocopherol as a non enzymatic antioxidants had a beneficial effect on the cumulus cell expansion and nuclear maturation of buffalo oocytes. Retinol supplementation yielded the best results.  
 

Keywords

Journal of Animal and Poultry Production
Volume 5, Issue 4
April 2014
Pages 211-220

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