Identification of FecxH and FecxG Mutations in Karadi Sheep Breed Using RFLP-PCR Technique in Sulaimani Province

Document Type : Original Article

Authors

Animal Science department, College of Agricultural Engineering Sciences, University of Sulaimani, Kurdistan Region, Iraq

Abstract

This investigation was led to recognize FecXH and FecXG changes in Karadi sheep. Right now Karadi sheep (4 single and 11 twining) were researched for the nearness of the FecXH and FecXG transformation utilizing PCR-RFLP. PCR items 240 and 141 bp was processed utilizing SpeI and HinfI limitation compound to identify FecXH and FecXG point Mutations, separately. The FecXH and FecXG DNA test demonstrated that there were no bearers for the FecXH and FecXG transformations in the chose Karadi sheep. These outcomes recommend that fruitfulness of this breed isn't connected to similar changes. In this way, it is important to look for different transformations or fertility qualities so as to create marker help choice methods and study the productive system of the Karadi breed.

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