Gabr, S., Shamiah, S., Elsanafawy, A. (2020). In Vitro Development of Vitrified Rabbit's Embryos as Affected by some Nutritional and Hormonal Treatments. Journal of Animal and Poultry Production, 11(7), 249-254. doi: 10.21608/jappmu.2020.108811
Sh. A. Gabr; Sh. M. Shamiah; Aya M. Elsanafawy. "In Vitro Development of Vitrified Rabbit's Embryos as Affected by some Nutritional and Hormonal Treatments". Journal of Animal and Poultry Production, 11, 7, 2020, 249-254. doi: 10.21608/jappmu.2020.108811
Gabr, S., Shamiah, S., Elsanafawy, A. (2020). 'In Vitro Development of Vitrified Rabbit's Embryos as Affected by some Nutritional and Hormonal Treatments', Journal of Animal and Poultry Production, 11(7), pp. 249-254. doi: 10.21608/jappmu.2020.108811
Gabr, S., Shamiah, S., Elsanafawy, A. In Vitro Development of Vitrified Rabbit's Embryos as Affected by some Nutritional and Hormonal Treatments. Journal of Animal and Poultry Production, 2020; 11(7): 249-254. doi: 10.21608/jappmu.2020.108811
In Vitro Development of Vitrified Rabbit's Embryos as Affected by some Nutritional and Hormonal Treatments
1Animal Production Department, Faculty of Agriculture, Tanta University.
2Biotechnology Depart., Animal Production Research Institute, Agricultural Research Center, Dokki,
Abstract
The purpose of the study was to measure the effect of 1-nutritional stages on reproductive performance in NZW rabbit doe. Total of 21 adult NZW rabbit were divided into 3groups (n=7). In the firstgroup does were given 100%NRC (Control,G1), In the second and third groups does were given 110(G2) or 120%(G3) from NRC for 3week before naturally mating. Weight, litter size and possibility rate at weaning and birth were recorded. 2-flushing (110% from NRC) (G1) and (superovulation, G2) using PMSG on ovarian activity, (recovery, freezability and in vitro development of rabbit embryos (n=4/group). Embryos were collected after 56h after naturally mating. Embryos freezing by vitrification and survival was assessed by in vitro culture. Results revealed that, 1-Total litter size and alive born at birth and litter size at weaning was much higher (P>0.05) in (Group2) than in other groups. 2-Mean number of corpus lutea and large follicles were much higher (P<0.05) in (Group2) than in (Group1) (8.5 and 6.25) also (6.25 and 4.5), respectively. Survival rate and normal embryos post-vitrification in (Group1) was higher than in (Group2) by 1.48% too 6.99% respectively. The percentage of hatched blastocyst stage in (Group1) was significantly (P<0.05) higher than in (Group2) (83.33 vs. 71.74%, respectively). However, expanded blastocyst stage showed opposite of this trend. Conclusion, improving reproductive performance for rabbit does in G2 (110% from NRC) than in other groups. Also the potential in vitro growthing of rabbit embryos from flushing group post-vitrified was significant higher than in hormonal group.
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