Dorra, T., EL. Sherif, K., Shamiah, S., Fouda, S. (2009). THE EFFECT OF VITRIFICATION PROCEDURES AND CRYOPROTECTANT TYPES ON POST-THAW SURVIVAL RATE OF RABBIT OOCYTES.. Journal of Animal and Poultry Production, 34(10), 9921-9930. doi: 10.21608/jappmu.2009.119365
Tork M. Dorra; Kh. EL. Sherif; Sh. M. Shamiah; Sara F. Fouda. "THE EFFECT OF VITRIFICATION PROCEDURES AND CRYOPROTECTANT TYPES ON POST-THAW SURVIVAL RATE OF RABBIT OOCYTES.". Journal of Animal and Poultry Production, 34, 10, 2009, 9921-9930. doi: 10.21608/jappmu.2009.119365
Dorra, T., EL. Sherif, K., Shamiah, S., Fouda, S. (2009). 'THE EFFECT OF VITRIFICATION PROCEDURES AND CRYOPROTECTANT TYPES ON POST-THAW SURVIVAL RATE OF RABBIT OOCYTES.', Journal of Animal and Poultry Production, 34(10), pp. 9921-9930. doi: 10.21608/jappmu.2009.119365
Dorra, T., EL. Sherif, K., Shamiah, S., Fouda, S. THE EFFECT OF VITRIFICATION PROCEDURES AND CRYOPROTECTANT TYPES ON POST-THAW SURVIVAL RATE OF RABBIT OOCYTES.. Journal of Animal and Poultry Production, 2009; 34(10): 9921-9930. doi: 10.21608/jappmu.2009.119365
THE EFFECT OF VITRIFICATION PROCEDURES AND CRYOPROTECTANT TYPES ON POST-THAW SURVIVAL RATE OF RABBIT OOCYTES.
1Poultry production Dept., Fac.of Agric., Man. Univ. Egypt.
2Animal production Dept., Res. Inst., Agric, Rec.Center,Giza,Egypt.
Abstract
Aim of the present study was to determine the effect of some factors affecting oocyte vitrification of rabbit does including, three cryodevices [Straws (S), Open pulled straws (OPS) and Sealed Open pulled straws (SOPS)] using three cryoprotectant types [40% ethylin glycol (EG), 40% dimethyl sulfoxide (DMSO) and their combination, 20% EG + 20% DMSO). Total of 36 New Zealand white (NZW) rabbit does with (5 mo old and 3.25±0.25 kg LBW) and were used in this study. Ovaries were collected and oocytes were harvested. The oocyte yield was recorded and the number of oocyte/ovary was counted, and oocyte recovery rate was calculated. Results indicated that total number of follicles averaged 36.0/doe and 18.0/ovary. Yield of oocytes averaged 31.3/doe and 15.5/ovary and oocyte recovery rate was 86.2%. Oocyte recovery rate was for 61.96, 11.65, 6.48, and 6.10% for compact, denuded, partial denuded and expanded oocytes, respectively. The corresponding frequency distribution was 71.89, 13.52, 7.52 and 7.07%, respectively. Relative to total number of vitrified oocytes, total survival rate of oocyte was not affected significantly by cryodevice, being 87.30, 83.09 and 85.77% for S, OPS and SOPS, respectively. Post-thaw survival rate of normal oocytes was the highest (68.33%, P<0.05) and abnormal oocytes was the lowest (17.44%, P<0.05) with SOPS and the opposite was obtained for straws (59.43 and 27.87%, respectively, P<0.05). While, OPS showed moderate values (63.31 and 19.78%, respectively), but did not differ significantly from each of straw and SOPS. Total survival rate of oocyte was not affected significantly by cryoprotectant type, being 85.38, 83.45 and 87.22% for EG, DMSO and their combination, respectively. Post-thaw survival rate of normal oocytes was the highest (72.56%, P<0.05) and abnormal oocytes was the lowest (14.66%, P<0.05) with EG+DMSO and the opposite was obtained for EG alone (56.92 and 28.46%, respectively, P<0.05). While, DMSO showed moderate values (61.97 and 21.48%, respectively).
In conclusion, using sealed open pulled straws and combination of 40% ethylin glycol and 40% dimethyl sulfoxide as cryoprotectant solution as vitrification procedures of rabbit oocytes showed the best results of post-vitrification survival rate.
View on SCiNiTO
Top