Home Articles List Article Information
Journal of Animal and Poultry Production
Journal Archive
Volume Volume 16 (2025)
Volume Volume 15 (2024)
Volume Volume 14 (2023)
Volume Volume 13 (2022)
Volume Volume 12 (2021)
Volume Volume 11 (2020)
Volume Volume 10 (2019)
Volume Volume 9 (2018)
Volume Volume 8 (2017)
Volume Volume 7 (2016)
Volume Volume 6 (2015)
Volume Volume 5 (2014)
Volume Volume 4 (2013)
Volume Volume 3 (2012)
Volume Volume 2 (2011)
Volume Volume 1 (2010)
Volume Volume 34 (2009)
Volume Volume 33 (2008)
Volume Volume 32 (2007)
Volume Volume 31 (2006)
Volume Volume 30 (2005)
Volume Volume 29 (2004)
Volume Volume 28 (2003)
Volume Volume 27 (2002)
Volume Volume 26 (2001)
Volume Volume 25 (2000)
El-Shamaa,, I., Metwally, A., Ibrahim, M., Abd El-Razek, I., El-Saidy, B., Al-Gindy, M. (2007). EFFECT OF SEPHADEX FILTRATION OF RAM SEMEN ON ITS FREEZABILITY AND FERTILITY. Journal of Animal and Poultry Production, 32(5), 3347-3358. doi: 10.21608/jappmu.2007.219516
I. S. El-Shamaa,; A. M. Metwally; M. A. Ibrahim; I. M. Abd El-Razek; B. E. El-Saidy; M. E. Al-Gindy. "EFFECT OF SEPHADEX FILTRATION OF RAM SEMEN ON ITS FREEZABILITY AND FERTILITY". Journal of Animal and Poultry Production, 32, 5, 2007, 3347-3358. doi: 10.21608/jappmu.2007.219516
El-Shamaa,, I., Metwally, A., Ibrahim, M., Abd El-Razek, I., El-Saidy, B., Al-Gindy, M. (2007). 'EFFECT OF SEPHADEX FILTRATION OF RAM SEMEN ON ITS FREEZABILITY AND FERTILITY', Journal of Animal and Poultry Production, 32(5), pp. 3347-3358. doi: 10.21608/jappmu.2007.219516
El-Shamaa,, I., Metwally, A., Ibrahim, M., Abd El-Razek, I., El-Saidy, B., Al-Gindy, M. EFFECT OF SEPHADEX FILTRATION OF RAM SEMEN ON ITS FREEZABILITY AND FERTILITY. Journal of Animal and Poultry Production, 2007; 32(5): 3347-3358. doi: 10.21608/jappmu.2007.219516

EFFECT OF SEPHADEX FILTRATION OF RAM SEMEN ON ITS FREEZABILITY AND FERTILITY

Article 3, Volume 32, Issue 5, May 2007, Page 3347-3358  XML PDF (497.13 K)
Document Type: Original Article
DOI: 10.21608/jappmu.2007.219516
View on SCiNiTO View on SCiNiTO
Authors
I. S. El-Shamaa,1; A. M. Metwally1; M. A. Ibrahim1; I. M. Abd El-Razek1; B. E. El-Saidy2; M. E. Al-Gindy2
1Department of Anim. Prod., Fac. of Agric. Kafrelsheikh Univ.
2Anim. Prod. Res. Inst., Agric. Res. Center, Dokki, Egypt
Abstract
Semen of 10 healthy mature crossbred (½ FR) rams was frozen in Tris-egg yolk glycerol extender before and after filtration through sephadex column G25-150 to investigate the effect of filtration on freezability and fertility outcomes during different seasons of the year. Filtration of semen through sephadex column resulted in significantly (P < 0.05) better maintenance of sperm progressive motility, live sperm count and sperm morphology compared to unfiltrated semen. There was significant (P < 0.05) decrease in sperm cell concentration x 109/ml after filtration (2.05 + 0.5) over the unfiltered semen (2.68 + 0.08). Progressive motility of filtered frozen-thawed spermatozoa was significantly (P < 0.05) improved to progressive motility of unfiltered frozen semen (60.10 v.s 43.88%). Conception rate was higher for ewes inseminated cervically with filtered frozen-thawed semen (63.4%) than those inseminated with unfiltered frozen semen (52.3%) at synchronized estrus with GnRH-PG-GnRH protocol. However, conception rate at observed estrus for ewes inseminated cervically with filtered frozen semen was (P < 0.05) better in comparison with those inseminated with unfiltered frozen semen (95 v.s 75%). These results concluded that removal of dead and abnormal spermatozoa by filtration of ram semen through sephadex column resulting in highest fertility after cervical artificial insemination.
Keywords
Ram; Semen; Filtration; frezability; fertility
Statistics
Article View: 115
PDF Download: 276