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El-Shamaa,, I., Metwally, A., Ibrahim, M., Abd El-Razek, I., El-Saidy, B., Al-Gindy, M. (2007). EFFECT OF SEPHADEX FILTRATION OF RAM SEMEN ON ITS FREEZABILITY AND FERTILITY. Journal of Animal and Poultry Production, 32(5), 3347-3358. doi: 10.21608/jappmu.2007.219516
I. S. El-Shamaa,; A. M. Metwally; M. A. Ibrahim; I. M. Abd El-Razek; B. E. El-Saidy; M. E. Al-Gindy. "EFFECT OF SEPHADEX FILTRATION OF RAM SEMEN ON ITS FREEZABILITY AND FERTILITY". Journal of Animal and Poultry Production, 32, 5, 2007, 3347-3358. doi: 10.21608/jappmu.2007.219516
El-Shamaa,, I., Metwally, A., Ibrahim, M., Abd El-Razek, I., El-Saidy, B., Al-Gindy, M. (2007). 'EFFECT OF SEPHADEX FILTRATION OF RAM SEMEN ON ITS FREEZABILITY AND FERTILITY', Journal of Animal and Poultry Production, 32(5), pp. 3347-3358. doi: 10.21608/jappmu.2007.219516
El-Shamaa,, I., Metwally, A., Ibrahim, M., Abd El-Razek, I., El-Saidy, B., Al-Gindy, M. EFFECT OF SEPHADEX FILTRATION OF RAM SEMEN ON ITS FREEZABILITY AND FERTILITY. Journal of Animal and Poultry Production, 2007; 32(5): 3347-3358. doi: 10.21608/jappmu.2007.219516

EFFECT OF SEPHADEX FILTRATION OF RAM SEMEN ON ITS FREEZABILITY AND FERTILITY

Article 3, Volume 32, Issue 5, May 2007, Page 3347-3358  XML PDF (497.13 K)
Document Type: Original Article
DOI: 10.21608/jappmu.2007.219516
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Authors
I. S. El-Shamaa,1; A. M. Metwally1; M. A. Ibrahim1; I. M. Abd El-Razek1; B. E. El-Saidy2; M. E. Al-Gindy2
1Department of Anim. Prod., Fac. of Agric. Kafrelsheikh Univ.
2Anim. Prod. Res. Inst., Agric. Res. Center, Dokki, Egypt
Abstract
Semen of 10 healthy mature crossbred (½ FR) rams was frozen in Tris-egg yolk glycerol extender before and after filtration through sephadex column G25-150 to investigate the effect of filtration on freezability and fertility outcomes during different seasons of the year. Filtration of semen through sephadex column resulted in significantly (P < 0.05) better maintenance of sperm progressive motility, live sperm count and sperm morphology compared to unfiltrated semen. There was significant (P < 0.05) decrease in sperm cell concentration x 109/ml after filtration (2.05 + 0.5) over the unfiltered semen (2.68 + 0.08). Progressive motility of filtered frozen-thawed spermatozoa was significantly (P < 0.05) improved to progressive motility of unfiltered frozen semen (60.10 v.s 43.88%). Conception rate was higher for ewes inseminated cervically with filtered frozen-thawed semen (63.4%) than those inseminated with unfiltered frozen semen (52.3%) at synchronized estrus with GnRH-PG-GnRH protocol. However, conception rate at observed estrus for ewes inseminated cervically with filtered frozen semen was (P < 0.05) better in comparison with those inseminated with unfiltered frozen semen (95 v.s 75%). These results concluded that removal of dead and abnormal spermatozoa by filtration of ram semen through sephadex column resulting in highest fertility after cervical artificial insemination.
Keywords
Ram; Semen; Filtration; frezability; fertility
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