EI-Dakar,, A., Gohar, Y. (2004). USE OF Bacillus subtilis IN MICROPARTICULATE DIETS FOR PRODUCING BIOSECURE Peneaus japonicus POSTLARVAE. Journal of Animal and Poultry Production, 29(12), 6853-6871. doi: 10.21608/jappmu.2004.239471
A. Y. EI-Dakar,; Y. M. Gohar. "USE OF Bacillus subtilis IN MICROPARTICULATE DIETS FOR PRODUCING BIOSECURE Peneaus japonicus POSTLARVAE". Journal of Animal and Poultry Production, 29, 12, 2004, 6853-6871. doi: 10.21608/jappmu.2004.239471
EI-Dakar,, A., Gohar, Y. (2004). 'USE OF Bacillus subtilis IN MICROPARTICULATE DIETS FOR PRODUCING BIOSECURE Peneaus japonicus POSTLARVAE', Journal of Animal and Poultry Production, 29(12), pp. 6853-6871. doi: 10.21608/jappmu.2004.239471
EI-Dakar,, A., Gohar, Y. USE OF Bacillus subtilis IN MICROPARTICULATE DIETS FOR PRODUCING BIOSECURE Peneaus japonicus POSTLARVAE. Journal of Animal and Poultry Production, 2004; 29(12): 6853-6871. doi: 10.21608/jappmu.2004.239471
USE OF Bacillus subtilis IN MICROPARTICULATE DIETS FOR PRODUCING BIOSECURE Peneaus japonicus POSTLARVAE
1Fish Resources and Aquaculture Department, Faculty of Environmental Agric. Sciences, Suez Canal Univ., EI-Arish. Egypt.
2Microbiology Division, Fac. of Science, Alex. Univ., Alexandria, Egypt
Abstract
Two separate feeding trails were carried out to study the effect of Bacillus subtilis in probiotic micro particulate diets on survival, growth and pathogen resistance of Penaeus japonicus larvae and postlarvae. The first one was done on an experimental scale while the second was done on a large scale as an application trail. Probiotic diet was formulated by adding 500 mg of Bacillus subtilis (7x107 cell/g) per 100 g basal diet that containing 53% protein with particles size of 350-800 IJm. In the experimental trail, 1000 postlarvae of P. japonicus at substage of PL1 were randomly distributed, stocked into six circular-conical bottom fiberglass tanks; each one had a capacity of 100-L. In the application trail, two 3.6 m3 U-shape fiberglass tanks were used to stock 180,000 hatched nauplii of P. japonicus in each. Survival of postlarvae fed probiotic diet was higher than those fed the basal diet. Enhanced growth was generally obtained in postlarvae fed probiotic diet compared with the basal diet in either experimental or application trails. Challenge of bacteria to pathogen, as a universal probiotic bacterium, was studied by evaluating its action against three different aquaculture bacterial pathogens, namely, Aeromonas hydrophila, Edwardsiella tarria, and Vibrio proteolyticus. A considerable change in the intra and extra-cellular proteins profiles of the three bacterial pathogens were observed when electrophoresed via SOS-PAGE techniques after mixing with B. subtilis. The proteolytic activity of the bacterial pathogens exhibited a sharp decrease when subjected to B. subtilis extra-cellular products. In addition, it was noted a positive effect of the extra-cellular products of B. subtilis against the pathogens and on reducing the antibiotic susceptibility when presented in culture water or in feed of shrimp.
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