The present study was conducted to demonstrate whether soy lecithin could be used as an alternative to egg yolk (EY) in tris-extender for cryopreservation of APRI rabbit semen. In tris-extender (E1), 18% EY was replaced by 1% (E2), 1.5% (E3), or 2% (E4) SBL (1:4). Semen was evaluated visually after cryopreservation stages and by CASA only post-thawing. Results showed that visual sperm motility, livability, abnormality, acrosome integrity, and membrane integrity were improved (P<0.05) by E3 after all cryopreservation stages. Slow, total progressive, and total motility were the highest (P<0.05), while non-motility and non-progressive motility were the lowest (P<0.05) with E3 compared with other extenders. In comparing with E1, E3 showed the best dynamic sperm parameters, in terms of the highest VCL, STR (P<0.05), VSL (P≥0.05), and the lowest (P<0.05) WOB. Prolificacy of rabbits showed the highest (6.68 kits/doe, P<0.05) by E3 compared with 4.96, 4.72, and 2.8 kits/doe for E1, E2, and E4, respectively. The correlation coefficient was the lowest (r=0.691, P<0.05) between acrosome integrity and litter size, and the highest (r= 0.921, P<0.001) between membrane integrity and prolificacy. Progressive motility and slow motility correlated with pregnancy (r=0.811and r=0.778, P<0.01) and prolificacy (r=0.898, P<0.001 and 0.801, P<0.01). In conclusion, addition of soybean lecithin at a level of 1.5% as an alternative to 18% egg yolk in tris extender may has a beneficial impact on motility parameters of cryopreserved APRI-rabbit spermatozoa, and prolificacy of doe rabbits.
Younan,, G., Shehab El-Din, A., Abdel-Khalek, A., El-Sherbieny, M., & Helmy, A. (2021). Soybean Lecithin as an Alternative to Egg Yolk in Tris-Based Extender of Cryopreserved Apri Rabbit Semen. Journal of Animal and Poultry Production, 12(1), 47-54. doi: 10.21608/jappmu.2021.149460
MLA
G. E. Younan,; A. M. Shehab El-Din; A. E. Abdel-Khalek; M. A. El-Sherbieny; Azza A. Helmy. "Soybean Lecithin as an Alternative to Egg Yolk in Tris-Based Extender of Cryopreserved Apri Rabbit Semen", Journal of Animal and Poultry Production, 12, 1, 2021, 47-54. doi: 10.21608/jappmu.2021.149460
HARVARD
Younan,, G., Shehab El-Din, A., Abdel-Khalek, A., El-Sherbieny, M., Helmy, A. (2021). 'Soybean Lecithin as an Alternative to Egg Yolk in Tris-Based Extender of Cryopreserved Apri Rabbit Semen', Journal of Animal and Poultry Production, 12(1), pp. 47-54. doi: 10.21608/jappmu.2021.149460
VANCOUVER
Younan,, G., Shehab El-Din, A., Abdel-Khalek, A., El-Sherbieny, M., Helmy, A. Soybean Lecithin as an Alternative to Egg Yolk in Tris-Based Extender of Cryopreserved Apri Rabbit Semen. Journal of Animal and Poultry Production, 2021; 12(1): 47-54. doi: 10.21608/jappmu.2021.149460
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