Eid, L., Shamiah, S., El-Regalaty, H., El-Keraby, F. (2011). SPERM DNA DAMAGE AND EMBRYONIC DEVELOPMENT AS RELATED TO FERTILITY POTENTIAL OF BUFFALO BULLS. Journal of Animal and Poultry Production, 2(5), 65-74. doi: 10.21608/jappmu.2011.83340
Laila N Eid; Sh. M. Shamiah; H. A. M. El-Regalaty; F. E. El-Keraby. "SPERM DNA DAMAGE AND EMBRYONIC DEVELOPMENT AS RELATED TO FERTILITY POTENTIAL OF BUFFALO BULLS". Journal of Animal and Poultry Production, 2, 5, 2011, 65-74. doi: 10.21608/jappmu.2011.83340
Eid, L., Shamiah, S., El-Regalaty, H., El-Keraby, F. (2011). 'SPERM DNA DAMAGE AND EMBRYONIC DEVELOPMENT AS RELATED TO FERTILITY POTENTIAL OF BUFFALO BULLS', Journal of Animal and Poultry Production, 2(5), pp. 65-74. doi: 10.21608/jappmu.2011.83340
Eid, L., Shamiah, S., El-Regalaty, H., El-Keraby, F. SPERM DNA DAMAGE AND EMBRYONIC DEVELOPMENT AS RELATED TO FERTILITY POTENTIAL OF BUFFALO BULLS. Journal of Animal and Poultry Production, 2011; 2(5): 65-74. doi: 10.21608/jappmu.2011.83340
SPERM DNA DAMAGE AND EMBRYONIC DEVELOPMENT AS RELATED TO FERTILITY POTENTIAL OF BUFFALO BULLS
Animal Production Res. Inst., Agric. Res. Center, Dokki, Giza, Egypt
Abstract
Conventional semen analysis are not sufficient to evaluate bull’s fertility, however it might be used as a preliminary evaluation for predicting fertility potential. Sperm DNA integrity may give a better evaluation of bull fertility potential. In the current study, six mature buffalo bulls were divided into two groups (based on a threshold of 70% motility; as high or low potential fertility). The first objective of the current study was to evaluate DNA fragmentation (measured as Comet value) in the two groups of bulls (three bulls/group) in relation to its potential fertility. The results showed that low potential fertility bulls had significantly (P<0.05) more sperm DNA damage, higher (P<0.05) sperm abnormal morphology and lower (P<0.05) sperm viability than that of sperm from the high potential fertility bulls.
Our second objective was to assess whether a relationship exists between bull’s sperm DNA damage and bull in vitro fertility. A significant variations (P<0.05) in the developmental competence of the embryos to the morula and blastocyst stages were observed. The results also showed a clear negative correlation between bull’s potential fertility and DNA integrity at and the ability of the fertilized ova to sustain its development to the morula (r=-0.756) and blastocyst stage (r=-0.643). These results indicate that measuring DNA integrity could be a powerful marker of bull’s fertility. Therefore, screening bulls for DNA damage should be emphasized along with routine semen analysis in selecting bulls either for natural mating or artificial insemination.
View on SCiNiTO
Top